Part:BBa_K4858005:Design
F2RL3 LAMP NEB1 FIP Primer
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Design Considerations: Several factors guided the design of the F2RL3 LAMP NEB1 FIP Primer. The primer was tailored to bind exclusively to the F2RL3 gene's region with the PAR4 mutation, ensuring that amplification is specific and reduces off-target effects. Given the importance of the SNP in our assays, the primer was designed to ensure the SNP's effective amplification. Considerations were made to ensure the SNP position was adequately captured between the primer's binding sites.
Source
The sequence for our F2RL3 LAMP NEB1 F3 Primer has its origins in the genomic sequence of the F2RL3 gene. We began our design process by extracting the relevant F2RL3 sequence from the Benchling platform. After pinpointing the desired sequence segment, especially emphasizing the region around the SNP of interest, we obtained a physical version of the primer through custom DNA synthesis services (IDT).
References
1. Hyman, L. B., Christopher, C. R., & Romero, P. A. (2022). Competitive SNP-lamp probes for rapid and robust single-nucleotide polymorphism detection. Cell Reports Methods, 2(7), 100242. https://doi.org/10.1016/j.crmeth.2022.100242 </p>
2. Biolabs, N. E. (n.d.). NEB LAMP Primer Design Tool. NEB LAMP. https://lamp.neb.com/#!/